Assessment of Genetic Stability of Micropropagated Bambusa balcooa Roxb. using RAPD Marker
نویسندگان
چکیده
Bambusa balcooa Roxb. was in vitro propagated by optimizing protocol using nodal segment from secondary branches with 100% success MS liquid media containing 100 mg/l Myo-inositol, 3% sucrose supplement 4.4 - 26.64 ?M BAP for shoot multiplication, and 2.69 32.26 NAA root induction. The highest multiplication (14.53 ± 0.33 folds), length (5.9 0.6 cm), number per explants (4.0 0.24), rooting (89.3 0.33%) obtained 13.32 (shooting) 26.88 (rooting) 1% aqueous leaf extract of Artemisia vulgaris L. (EAV). Twenty RAPD (Random amplified Polymorphic DNA) primers were used individually to amplify DNA tissue culture-raised plants the mother plant where 8 yielded monomorphic banding patterns reproducible, clear, scorable bands (2.8 primer) ranging 250 1800 bp respectively which revealed micropropagated B. retained their genetic stability.
 Plant Tissue Cult. & Biotech. 31(1): 81-95, 2021 (June)
منابع مشابه
assessment of the efficiency of s.p.g.c refineries using network dea
data envelopment analysis (dea) is a powerful tool for measuring relative efficiency of organizational units referred to as decision making units (dmus). in most cases dmus have network structures with internal linking activities. traditional dea models, however, consider dmus as black boxes with no regard to their linking activities and therefore do not provide decision makers with the reasons...
Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers
Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68...
متن کاملautomatic verification of authentication protocols using genetic programming
implicit and unobserved errors and vulnerabilities issues usually arise in cryptographic protocols and especially in authentication protocols. this may enable an attacker to make serious damages to the desired system, such as having the access to or changing secret documents, interfering in bank transactions, having access to users’ accounts, or may be having the control all over the syste...
15 صفحه اولGenetic Stability of Micropropagated Plantlets in Date Palm
Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (R1-6) and mother plant in Iranian date palm (Phoenix dactylifera L.) cultivar Khanizi. Total genomic DNA extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide Fermantas primers. Four primers of...
متن کاملAnalysis of toxic, antidiabetic and antioxidant potential of Bambusa balcooa Roxb. leaf extracts in alloxan-induced diabetic rats
Bambusa balcooa (Poaceae) is native to India and has been used traditionally by the tribes of Northeast India to treat diabetes. The present investigation was aimed to evaluate the toxicity, anti-diabetic activity along with in vitro antioxidant activity of the leaf of B. balcooa in alloxan-induced diabetic rats and also identify active compounds by using HPLC. The acute toxicity test of aqueou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2021
ISSN: ['1818-8745', '1817-3721']
DOI: https://doi.org/10.3329/ptcb.v31i1.54114